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2 edition of Plasmid-mediated tetracycline resistance in escherichia coli k-12. found in the catalog.

Plasmid-mediated tetracycline resistance in escherichia coli k-12.

Clare Alison Lowery

Plasmid-mediated tetracycline resistance in escherichia coli k-12.

by Clare Alison Lowery

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  • 3 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Birmingham, Dept of Genetics.

ID Numbers
Open LibraryOL13796075M

Silver resistance of sensitive Escherichia coli J53 and resistance plasmid-containing J53(pMG) was affected by halides in the growth medium. The effects of halides on Ag1 resistance were measured with AgNO 3 and silver sulfadiazine, both on agar and in liquid. Low concentrations of Cited by:   The aim of this study was to investigate the prevalence and transferability of resistance in tetracycline-resistant Escherichia coli isolates recovered from beef cattle in South Korea. A total of E. coli isolates were collected from feces in South Korea, and were confirmed to be resistant to tetracycline. The tetracycline resistance gene tet (A) (%) was the most prevalent, followed Cited by:

Emergence of plasmid-mediated colistin resistance (MCR-1) among Escherichia coli isolated from South African patients The polymyxin antibiotic colistin is an antibiotic of last resort for the treatment of extensively drug-resistant Gram-negative bacteria, including carbapenemase-producing Enterobacteriaceae. Ball PR, Shales SW, Chopra I. Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic. Biochem Biophys Res Commun. Mar 13; 93 (1)– Beck CF, Mutzel R, Barbé J, Müller W. A multifunctional gene (tetR) controls Tnencoded tetracycline resistance. J Bacteriol. May; (2)

Antibiotic resistant bacteria and plasmid-mediated quinolone resistance genes and ampC gene were investigated for Escherichia coli isolates from two typical municipal wastewater treatment plants in both dry and wet seasons by using the antibiotic susceptibility test and PCR assay, respectively. The results showed that % of the isolates () were found resistant to antibiotic(s) tested. In this study, the prevalence of the plasmid-mediated quinolone resistance (PMQR) genes among pediatric clinical isolates of Escherichia coli and Klebsiella pneumoniae was investigated. A total of nonduplicate clinical isolates of E. coli and K. pneumoniae collected between January and December from Beijing, China, were by: 7.


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Plasmid-mediated tetracycline resistance in escherichia coli k-12 by Clare Alison Lowery Download PDF EPUB FB2

Vol. 93, No. 1, Ma BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Pages PLASMID-MEDIATED TETRACYCLINE RESISTANCE IN ESCHERICHIA COLI INVOLVES INCREASED EFFLUX OF THE ANTIBIOTIC P.R.

Ball, S.W. Shales and I. Chopra Department of Bacteriology The Medical School, University of Bristol, Bristol BS8 1TD Cited by: Franklin, T.J.: Resistance to Escherichia coli to tetracycline. Changes in permeability to tetracyclines in Escherichia coli bearing transferable resistance factors.

Biochem. Cited by: Journals & Books; Register Sign in. Vol Issue 1, 13 MarchPages Plasmid-mediated tetracycline resistance in escherichia coli involves increased efflux of the antibiotic.

Author links open overlay panel P.R. Ball S.W. Shales I. Chopra. Show by: The ecological impact of antibiotic resistance in the absence of selective pressure has been poorly studied. We assessed the carriage of tetracycline resistance genes, persistence in the microbiota, fecal population counts and virulence factor genes in commensal, intestinal Escherichia coli strains obtained from Swedish infants followed during the first year of life with regular Cited by: Chuit CF, Pitton JS.

Non-transferable tetracycline resistance in Escherichia coli K Chemotherapy. ; 14 (4)– Clewell DB, Helinski DR. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Proc Natl Acad Sci U S A. Apr; 62 (4)–Cited by: 2. Probing the expression of plasmid-mediated tetracycline resistance in Escherichia coli, p. – In D. Schlessinger (ed.), Microbiology— American Society for Microbiology, Washington, by: 8.

Expression of eight transporter genes of Escherichia coli K and its ΔacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR.

All transporter genes were overexpressed after induced resistance with acrF being fold more expressed in the ΔacrAB tetracycline-induced Cited by: of antibiotic resistance in bacteria isolated from humans.

Th e prudent use of tetracycline antibiotic in poultry production is essential as well as per-manet monitoring of the presence of the tetracycline resistance.

Key words: Escherichia coli, poultry, resistance, tetracycline 1 Corresponding author: [email protected] Size: KB. A total of Escherichia coli isolates from raw and treated municipal wastewaters were investigated to evaluate the frequency and persistence of antibiotic resistance and to detect the occurrence of conjugative R plasmids and integrons.

The highest resistance rates were against ampicillin (%), tetracycline (%), sulfamethoxazole (%) and streptomycin (%).Cited by: The arrival of a plasmid in a new bacterium usually produces a fitness cost (reviewed in).Therefore, unless the plasmid is able to spread horizontally fast enough to ensure survival as a pure genetic parasite, theory would predict its removal from the bacterial population ().Thus, in the absence of selection for plasmid-encoded traits, the costly plasmid will be purged from the population by Cited by: The conjugative plasmid pUR determines tetracycline resistance and enables cells of Escherichia coli K to utilize sucrose as the sole carbon source.

Three types of mutants affecting sucrose metabolism were derived from pUR One type lacked a Cited by: The conjugative plasmid pUR determines tetracycline resistance and enables cells of Escherichia coli K to utilize sucrose as the sole carbon source. Three types of mutants affecting sucrose metabolism were derived from pUR One type lacked a specific transport system (srcA); another lacked sucrosephosphate hydrolase (scrB); and the third, a regulatory mutant, expressed both of Cited by: The transferability of a large plasmid that harbors a tetracycline resistance gene tet(S), to fish and human pathogens was assessed using electrotransformation and conjugation.

The plasmid, originally isolated from fish intestinal Lactococcus lactis. We coauthored reports of a urinary tract infection due to an Escherichia coli strain coexpressing plasmid-mediated carbapenemase and colistin resistance genes and a series of MCR-1–positive isolates among a retrospective collection (–) of E.

coli isolates from diarrheic veal calves in by: Resistances that observed against Tetracycline was closed with Islam et al. [18], they showed % resistance to Tetracycline of Escherichia coli isolated from poultry farm at Chittagong District.

Plasmid-mediated resistance to the aminoglycoside antibi- otics gentamicin, tobramycin, and kanamycin has been clearly established in these strains (10, 17), and we have.

To the best of our knowledge, this is the first time that putative environmental levels of levofloxacin (LEV) have been shown to facilitate the dissemination of antibiotic-resistant E. coli via plasmid-mediated transformability.

These findings will promote our understanding of transformation-induced development of antibiotic resistance upon Author: Hai-yan Wu, Dan-yang Shi, Dong Yang, Jing Yin, Zhong-wei Yang, Jun-wen Li, Wu Yang, Min Jin. Levels of resistance to tetracycline, chlortetracycline, demethylchlortetracycline, doxycycline, oxytetracycline, methacycline, pyrrolidinotetracycline, minocycline, and beta-chelocardin of Escherichia coli K carrying transposon Tn10 or defined DNA segments of Tn10 were determined.

In all cases, tetA was the only gene required for by: 3. Antibiotic resistance in Escherichia coli A practical investigation of bacterial conjugation u Annie Darwin, who died at the age of ten, probably from tuberculosis.

Darwin one of the first photographs of a micro-organism. Charles replied: “I well remember saying to myself between twenty and thirty years ago, that if ever theFile Size: 1MB. Abstract. Escherichia coli is the most common Gram-negative bacterial pathogen, presenting both a clinical and an epidemiological challenge.

In the last decade, several successful multidrug-resistant high-risk strains, such as strain E. coli ST have evolved, mainly due to the growing selective pressure of antimicrobial use.

These strains present enhanced fitness and Cited by: 2. The strain identified as mcr-1 positive was genotyped and its antibiotic susceptibility was established. The mcr-1 containing plasmid was mobilized into Escherichia coli K and its sequence was determined.

Results: A single E. coli isolate (OM97) carrying mcr-1 gene was identified, while no strains carrying the mcr-2 gene was by: 7. Ball PR, Shales SW, Chopra I () Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic.

Biochem Biophys Res Commun – doi: /SX(80) PubMed CrossRef Google ScholarAuthor: Xian-Zhi Li, Hiroshi Nikaido.The tet system, based on the regulatory elements of the Escherichia coli tetracycline resistance operon (Hillen et al., ; Hillen and Berens, ), is the most popular system to control gene bacteria, transcription of the genes mediates resistance to tetracycline and is under the control of the protein TetR (Tet repressor) that binds to the tetO (operator) within the operon.